Do I need to release my game on Google play store to show ad?

Hi all, I have only started learning to use unity for game development.
I am making a game for android platform and I have followed the following links in making a button to show ad:

https://unityads.unity3d.com/help/monetization/integration-guide-unity
https://docs.unity3d.com/ScriptReference/Advertisements.Advertisement.Show.html

I have enabled test mode on the editor and it worked (the test ad said everything was working correctly).

But when testing the game on...

Do I need to release my game on Google play store to show ad?